Xypex Bio-San C500 is a uniquely designed admixture for integral, long-term protection of concrete in harsh sewage conditions with levels of H2S that cause microbial induced corrosion (MIC). Bio-San C500 combines potent antimicrobial protection along with the unique crystalline technology of the Xypex Admix C-Series.

Bio-San contains bio-active mineral solids that become permanently fixed within the cement matrix impairing bio-film formation thus inhibiting the growth of acid causing sewer bacteria such as Thiobacillus due to concentrations of H2S. The unique Xypex Crystalline Technology creates a permanent structure throughout the pores and capillary tracts providing waterproofing and enhanced chemical protection including acid and sulphate resistance. Bio-San C500 prevents microbial induced corrosion, stops infiltration/exfiltration of water, and provides acid and sulphate resistance, significantly extending the service life of concrete sewage collection systems and waste water infrastructure.

Product Data
Bio-San C500 VIEW PDF
Brochure VIEW PDF

Advantages of Xypex

  • Inhibits microbial induced corrosion
  • Resists extreme hydrostatic pressure
  • Resistant to aggressive chemicals (acids and sulphates)
  • Can seal static hairline cracks up to 0.5 mm
  • Becomes a permanent, integral part of the substrate
    and cannot be punctured, damaged or lose adhesion
  • Does not contain any VOCs
  • Less costly to apply than most other methods
  • Added to the concrete at the time of batching and therefore
    not subject to weather and surface moisture constraints

Recommended for

  • Manholes / Sewer Pipes
  • Pump and Lift Stations
  • Head Works
  • Septic Tanks
  • Digesters
  • Clarifiers
  • Industrial Structures


Xypex Bio-San C500 is packaged in 50 lb. (22.7 kg) pails. Contact the manufacturer for availability of customsized packaging to meet the requirements of your specific project.

Dosage Rate

1% by weight of total cementitious content.


Xypex products must be stored dry at a minimum temperature of 45ºF (7ºC). Shelf life is one year when stored under proper conditions.

Bio-San C500 Specific Details

Filling Tie Holes in Xypex Bio-San C500 Concrete – Close + Details VIEW FILE

Xypex recommended procedures for waterproofing concrete tie holes in new construction where Xypex Bio-San C500 concrete has been used to protect against MICC attack.

Crack Repair in Xypex Bio-San C500 Treated Concrete – Close + Details VIEW FILE

Xypex recommended procedure for crack repairs performed on the sewage side of concrete elements that contain Xypex Bio-San C500.

Repair of Defective Bio-San Concrete, Rock Pockets or Honeycombs – Close + Details VIEW FILE

Xypex recommended procedure for repair of standard, small or medium-large sized deficiencies in Xypex Bio-San C500 treated concrete.

General Resources

Product Data VIEW FILE
Specifications – Bio-San C500 VIEW FILE

Pinheiros Sewage Pumping Station

São Paulo, Brazil

Xypex Bio-San C500 was selected due to its ability to permanently waterproof the concrete and increase the structure’s resistance to sulfates and sulfuric acid attacks while preventing the growth of the bacteria that causes MIC.

Project Sheet
Project Sheet VIEW PDF

Dresden Wastewater Treatment Plant Upgrade

Dresden, USA

The designer was concerned about protection of the concrete elements in this tank from the destructive processes associated with microbial induced corrosion. To protect against this Xypex Bio-San C500 antimicrobial admixture was chosen to be added to the concrete mix.

Project Sheet
Project Sheet VIEW PDF

Sanofi Pasteur Toronto Water & Wastewater Management Facility

Toronto, Canada

Consulting with the Xypex team, the engineers and contractors determined that using a Xypex system would be the ideal solution to protect and waterproof the new concrete structures.

To achieve this, Xypex Admix C500 and Xypex Bio-San C500 replaced the traditional coating specified for this job.

Both Xypex Admix C-500 and Bio-San C500 are added to the concrete mix at the time of batching, eliminating the need to apply a coating at a later date.

These Xypex products react with cement and the byproducts of cement hydration to fill the capillaries in the internal structure of the concrete, creating a non-soluble crystalline matrix to waterproof the concrete and protect it from chemical attack throughout its useful life. 

Project Sheet
Project Sheet VIEW PDF

Lambeau Field

Green Bay, Wisconsin, USA

By selecting Xypex Bio-San C500, the general contractor, Miron Construction Company Inc, saved time and money as this product provides comprehensive waterproofing and MIC resistance without any extra drying, curing or installation time. 

With the Xypex Bio-San integrated into the concrete, the concrete is protected for the structure’s service life.


Project Sheet
Project Sheet VIEW PDF

Largo Wastewater Treatment Plant

Florida, USA

The concrete structures found in most wastewater treatment plants are subject to some of the harshest environments imaginable, including abrasion erosion, chemical attack, chloride ion-induced corrosion, freeze-thaw conditions, and microbiologically induced corrosion (MIC). Large cracks were power-chiseled to create a space to accept a dry-pack of Megamix II with Bio-San, which was then covered with a top coat of Megamix II with Bio-San. The Xypex repair mortar can be applied via sprayer or trowel at a thickness of 3/8 to 2 inches. It must be applied to a surface in saturated surface dry (SSD) condition.

Project Sheet
Project Sheet VIEW PDF

Projects Using This Product

Wastewater Collection & Treatment

River Islands Sewage Pump Station


The River Island Sewage Pump Station faced two major challenges: as a sewage pump station the concrete required protection from microbial induced corrosion (MIC); and 9 m (30 ft) of the 12 m (40 ft) deep structure would be immersed in the surrounding water table. Xypex Bio-San C500 admixture was  chosen by the City of Lathrop as an alternative to the specified system.

Wastewater Collection & Treatment

South Lathrop Commons Center Sewage Pump Station


In order to protect the concrete structure from microbial induced corrosion and to achieve water tightness, Xypex Bio-San C500 was added to the concrete mix at the Jensen Precast’s plant at the time of batching. The Bio-San C500 contains antimicrobial crystalline technology which both kills the acid-producing microbes which proliferate in sewage structures as well as providing watertight, chemically resistant concrete with crack-healing properties.

Wastewater Collection & Treatment

Chatham Park WWTP


Due to the potentially high levels of H2S in the Bioreactor McKim & Creed, the engineers of record specified the use of a high build acid resistant coating to protect the concrete. Bowen Engineering proposed to the engineers Xypex Bio-San C500 as an alternate to this coating because it contains Xypex’s antimicrobial crystalline technology.

Wastewater Collection & Treatment

Fairchance Sewer Line Extension


Liners or coatings would normally be used to perform this function. As these are expensive and time consuming to install, the engineers decided to specify the inclusion of Xypex Bio-San C500 admixture in the pre-cast concrete.

Wastewater Collection & Treatment

Sonic Sanitary Lift Station & Manholes – Arizona WWTP


The Arizona Lift Station project involved a large cast-in-place wet well and drop/ discharge precast manholes. Xypex Bio-San C500 was incorporated into all cast-in-place concrete and grout in direct or indirect contact with wastewater.

Wastewater Collection & Treatment

American Furniture Manholes


The design team was looking for corrosion protection for the manholes and chose Xypex Bio-San C500 over the alternate antimicrobial admixture. Xypex Bio-San C500 was chosen because it provides the precast sewage elements with protection against microbial induced corrosion and aggressive chemicals, as well as waterproofing and crack healing properties.